Evaluation of Biochemical Markers in Hepatocyte like Cells Differentiated from Adipose Derivation Stem Cells

Recent evidence proposes that cells with the properties of mesenchymal stem cells (MSCs) can be extracted from adult peripheral tissues, including muscle, dermis and adipose tissue. Adipose tissue is ubiquitous and easily obtainable in large quantities. We separated Adipose derived stem cells (ADSCs) from part of subcutaneous adipose tissue from rat. These cells could be readily differentiated into cells of the chondrocyte, osteocyte, hepatocyte and adipocyte lineage demonstrating their multipotency. It is necessary to describe the ADSCs surface expression of the typical markers by using flow cytometric and survey the results. Finally, these cells transformed into hepatocyte like cell. Immunocytochemical analysis demonstrated that ADSCs are capable to express Albumin and -fetoprotein during differentiation. Moreover, we determined biochemical markers of hepatocyte like cells such as SGPT, SGOT, urea Production and glycogen synthesis. At last we isolated total RNA from hepatocyte like cells at shown days after differentiation and performed RTPCR by using the specific primers (albumin, Fetoprotein and Alpha 1-Antitrypsin).